ELISA Assays-

Cynomolgus Macaque (Macaca fascicularis) IgM

Catalog No.: 53414
Turnaround Time: 3-5 Days
Cross-reactivity: Human, Baboon, Rhesus Macaque; does NOT cross react with Bovine or Porcine
Method: Enzyme-linked Immunosorbant Assay (ELISA)
Performance:

  • Sensitivity = 0.1 mg/mL
  • Intra-assay CV = 2.6%
  • Inter-assay CV = 18%
  • Analytical Range = 0.1 - 10 mg/mL
  • Recovery Studies = 92.4%

Reference Ranges:

  • Male = 2.3 - 3.9 mg/mL
  • Female = 0.8 - 3.2 mg/mL
  • General Population = 0.8 - 3.9 mg/mL

Application: The IgM quantitation provides the level of IgM present in a given volume of cynomolgus serum or plasma. This assay is currently available for baboons, cynomolgus macaques, and rhesus monkeys. To request validation in another species, please contact us via phone or email, or submit an online inquiry.

Sample Requirements: Please submit a minimum of 0.5mL of frozen sample. For accurate results sample must remain frozen throughout transit. Contact Xeno Diagnostics for further information on alternative sample sizes.

Stability: 107% through 3 freeze/thaw cycles, store at -20°C for up to 1 year

View Cynomolgus Macaque IgM Quantification Tech Spec Sheet (Printable Version)

____________________________________________________________________________________________

Structure: IgM is a 900 kDa pentavalent protein. The molecule possesses five variable regions each composed of light and heavy chains. IgM is the first immunoglobulin produced upon antigenic stimulation. The variable region imparts unique binding specificity while the heavy chain Fcµ constant region provides functional characteristics of the isotypic class.

Production: Plasma cells synthesizes IgM after antigenic stimulation. The IgM synthesized will recognize and bind to the specific stimulating antigen.