Mixed Lymphocyte Reactions (MLR)

Mixed Lymphocyte Reactions (MLR’s) provide researchers a tool to evaluate the immune response of a recipient to implanted tissues or devices. Lymphocytes isolated from the recipient are stimulated with non-proliferative lymphocytes isolated from the donor and the proliferative response is measured. Xeno Diagnostics utilizes DNA incorporation of BrdU to qualitatively determine the amount of cell proliferation. To ensure that only the proliferation of the recipient’s lymphocytes is being evaluated, the stimulator cells are rendered non-proliferative (“NP”) by treatment with a DNA crosslinker. The Mixed Lymphocyte Reaction can be used to evaluate:

  • Autologous Response: Recipients cells vs. “NP” Recipient Cells
  • Allogeneic Response: Recipients cells vs. “NP” cells of different individuals of the same species
  • Xenogeneic Response: Recipient cells vs. “NP” cells from individuals of a different species
  • Mitogenic Response: Recipient cells stimulated with a mitogen (used as a positive control)

 Longitudinal Mixed Lymphocyte Reaction testing is available for non-human primates (baboon, cynomolgus macaque, rhesus monkey) for the evaluation of tissue and device implants. If you still have questions regarding this and other cell-based assay services, contact us at (317) 973-4079, or by filling out the form below. If you have more questions about MLR’s, see our Mixed Lymphocyte Reaction FAQ page, to learn more about our contract research services, see our capabilities page.