Cryopreservation Services

Why trust Xeno Diagnostics?

Xeno Diagnostics has over 15 years of experience performing custom immunological cell-based assays in support of clinical trials and cell therapy product development. Cell cryopreservation has been an integral component of our business as a result. We have worked with a wide variety of cellular materials from both human and non-human sources; as such, we have expanded our cryopreservation capabilities to include not just freezing services, but services to ensure the cellular material retains viability and activity after recovery. Activity assessments are tailor fit to the intended use of the material – whether it be analysis of T-cell reactivity, or in-vitro efficacy and dose-response of a particular cellular therapy.    

In addition, to support our other cell-based work, we have developed our own donor and biobank program for collection and cryopreservation of PBMCs for internal research as well as external use. Our repeat donors, which are pre-screened for allogeneic activity and HLA-typed, are a reliable cell source for client projects where continuity in assay performance needs to be maintained over the project lifetime (months to years). These cells are also available to our clients who wish to continue testing outside of our laboratory.

Fast and Reliable and Clinical Trial Support

Our decades of experience isolating and cryopreserving PBMCs provides a level of comfort and assurance to clients looking for a service provider to cryopreserve, hold, and ship their cells. We have provided this service to numerous past and present clients in support of clinical trials. Centrally located in Indianapolis, IN, a major shipping hub for the Midwest, allows us to receive timely shipments by both air and ground. Our facility routinely processed and preserved specimens within 8 hours of collection at the clinical site, or at a maximum of 24 hours for specimens shipped overnight from other areas of the country. Contact us to speak with a member of our cryopreservation team to see how Xeno Diagnostics can help you.

Safe and Secure Storage

Xeno also has professional storage and distribution services, from -80° C freezers for short-term cell storage, as well as serum/cell lysates, to liquid nitrogen (liquid or vapor phase) for long-term banking purposes. Our facilities are monitored 24/7/365 for security and environmental conditions (temperatures, humidity, and power), with real-time monitoring in the event of an emergency. We have on-site power backup for full facility, providing an extra level of security. Routine QC assessments of our freezers and tanks are performed in an effort to identify any potential problems before they become critical. We utilize professional cryo-courier services for distribution purposes.

Protocol Development Services

In addition to applying standard methods for cell cryopreservation, we have the ability to develop improved methods for hard-to-freeze cells. Cryopreservation entails exposing cells to numerous extreme conditions and understanding how cells respond to each of these extremes is challenging. Yet, this level of understanding is crucial for overcoming the challenges with recalcitrant cells.

Xeno has doctoral-level cryobiologists on staff with decades of experience in developing improved methods for cell cryopreservation in both research and clinical settings. Partner with Xeno to improve your cryopreservation protocols and move your research or development efforts forward in a robust manner. Contact us today to get started.

Functionality Assessment Services

Historically, post-thaw assessment has been performed by relatively simple viability assessments (trypan blue exclusion, for example). While this may be a good first-pass assessment of cryo-survival, a more critical assessment of cellular function is necessary to determine if the cells truly were preserved. Xeno has experience with numerous cell-based assays that can be leveraged to determine the health and robustness of a post-thaw cell population. Such assays include:

• ELISpot
• Phenotyping (surface and intracellular markers)
• T-cell Activation Assays
• Cytokine Release Assays
• Custom functional cell-based assays – unique to the mechanism of action of the product or cellular therapy