Mixed Lymphocyte Reactions

The Mixed Lymphocyte Reaction (MLR) can be used to evaluate autologous, allogeneic, and xenogenic reactions. At Xeno, our MLR’s are customized to address our clients’ questions of interest. This may include proliferation assays or lymphocyte subset analysis.

MLR’s measure T-cell response from one donor that is stimulated by antigen presenting cells (APC’s) from a second donor, due to an HLA mismatch. This stimulation is often measured by cell proliferation, but can also be evaluated by cytokine expression.

The MLR is a useful tool in evaluating immunomodulatory drugs and implantable materials. One can compare the immune response of an allogeneic donor pair in the presence of the test material to the baseline response (absence of the material).

MLR’s should be optimized for different test materials. This can include using different donor pairs, varying the cell concentrations and ratios, incubation times, MTP format, positive, negative, and vehicle controls, test material concentrations, and more. At Xeno, we create bespoke MLR’s that provide reliable and consistent results for each client’s specific needs.

We have created additional resources below to address common questions concerning MLR’s:

If you are in need of a service provider for an MLR, or need additional information, please see the following resources: