Transplant Immunology

The immune system may prevent a successful transplantation by recognizing the foreign HLA molecules on transplanted organs or cells. Because the major driver of rejection is immune responses to transplanted grafts, it is important to identify recipient’s immunity accurately in clinical transpltheantation. We have established several immunological techniques to support the advances in transplantation immunology with extensive experience using human, baboon, bovine, and porcine cells.

Mixed Lymphocyte Reactions

The MLR is used to evaluate the proliferative responses of T-cells when co-cultured with clients test articles. Our suite of MLRs is highly customizable with one-way/two-way, autologous, allogeneic, xenogeneic and mitogenic assays available. Through the use of Xeno Diagnostics human donor bank, client products are tested with donor PBMC pairs having known immunoreactivity.

Immunoglobulin Testing

  • Total Serum IgG and IgM Screening
  • IgG/IgM specific immunoassays can be performed by
  • ELISA or Flow Cytometry

Cytotoxicity Assays

  • Antibody Mediated Cytotoxicity
  • Complement Dependent Cytotoxicity

Immunophenotyping

Modified cells can be phenotyped for classic immunology cell markers using our flow cytometry capabilities.

Macrophage Phagocytosis

To determine a test articles affect on macrophage phagocytosis, macrophages are co-cultured with the article of interest. Phagocytosis of macrophages activated by bacteria (E.coli conjugated fluorochrome) or latex particles in the test system is measured by flow cytometry.

Alpha-gal Detection

The αGal epitope (Gal α1-3Galβ1-4GLcNAc-R) is unique to mammals and widely expressed on many mammalian cells. The Non-Human Primate αGal IgG (H & L) and Non-Human Primate αGal IgM (mu chain) ELISA provide a rapid and convenient method to monitor the expression of αGal IgG and IgM antibodies in a variety of non-human primate species. The level of antibody production is paired with the initial (time point 0) sample, and the level of relative fold increase is reported.

Custom Transplant Immunology Assays

Please contact us if you require a custom project that utilizes our technical capacity or a modification to the assays above to meet your research efforts.

Technical Capacity/Technologies Utilized:

  • Standard and custom ELISAs
  • 13 color Flow Cytometry
  • Luminex Multiplex Technology

Frequently Asked Questions

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