The immune system may prevent a successful transplantation by recognizing the foreign HLA molecules on transplanted organs or cells. Because the major driver of rejection is immune responses to transplanted grafts, it is important to identify recipient’s immunity accurately in clinical transpltheantation. We have established several immunological techniques to support the advances in transplantation immunology with extensive experience using human, baboon, bovine, and porcine cells.
Modified cells can be phenotyped for classic immunology cell markers using our flow cytometry capabilities.
To determine a test articles affect on macrophage phagocytosis, macrophages are co-cultured with the article of interest. Phagocytosis of macrophages activated by bacteria (E.coli conjugated fluorochrome) or latex particles in the test system is measured by flow cytometry.
The αGal epitope (Gal α1-3Galβ1-4GLcNAc-R) is unique to mammals and widely expressed on many mammalian cells. The Non-Human Primate αGal IgG (H & L) and Non-Human Primate αGal IgM (mu chain) ELISA provide a rapid and convenient method to monitor the expression of αGal IgG and IgM antibodies in a variety of non-human primate species. The level of antibody production is paired with the initial (time point 0) sample, and the level of relative fold increase is reported.