Migration Assays

Migration Logo

Recruitment and chemotaxis of immune cells towards the tumor microenvironment (TME) is an important metric of cell-function. TME differs from the environment around normal healthy tissue in that it is often hypoxic, acidic, and with inadequate blood flow, thus leading to the buildup of toxic cell byproducts along with the release of anti-inflammatory chemokines. These conditions result in the tolerization of immune cells as well as the polarization of T-cells and macrophages to immune suppressing T-regs and M2 macrophages, respectively. The ability of pro-inflammatory immune cells to overcome these suppressive effects and migrate towards and infiltrate tumors is of critical importance to effective immunotherapy.

Xeno Diagnostics offers several assays to test immune cell function in an in-vitro assay using donor human PBMCs with client test articles.

  • Cell Migration Assay – assess therapeutic enhanced chemotaxis
    • Chemokine attractants released by cancer cells can initiate migration of immune cells (T-cells, NK cells, and macrophages) to migrate along the chemokine gradient. However, this effect is offset as the tumor microenvironment suppresses immune cell function in order to “hide” from our immune system. This trans-well migration assay is designed to test the ability of new immunotherapies to recover immune function and initiate recruitment of pro-inflammatory cells.

Xeno Diagnostics is a CLIA certified high complexity lab and adheres to GLP and GCLP standards. We have had years of experience helping clients navigate the regulatory landscape and produce high-quality assays, documentation, and reporting to fit the regulatory requirements for each stage of product development.