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Evaluation of a compounds effect on the inhibition of cell proliferation is performed with human peripheral blood derived mononuclear cells (PBMC). Cells are isolated and incubated with the compounds of interest for 4 – 7 days and proliferation assessed through measurement of BrdU incorporation into the DNA of actively proliferating cells. Representative studies using either human PBMCs less than 24 hours old (fresh) or thawed cryopreserved PBMCs (frozen) in the presence of titered concentrations of dexamethasone are depicted below.
Assessment of diluent inhibition of proliferation is a common cause for concern. A representative study evaluating the effect on human PBMC proliferation with increasing concentrations of DMSO, a common diluent for many compounds, with 0.1 uM Dexamethasone (as compound of interest) is shown in the following graph.
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